IN VITRO ANTI BACTERIAL STUDY OF ANANDBHAIRAV RAS BY SELF CREATED FOOD POISONING

Dr. Savita Saini* and Dr. Sharad Maroti Porte

ABSTRACT

In Ayurveda infectious diseases and their epidemics have been discussed under the heading of Aupsargic Vyadhi and Janpadodhvans respectively. Abundant of herbal, mineral and herbomineral preparations have found in Ayurveda which have being continuously using for infectious disease by Ayurvedic practiontioer. Anand Bhairav Ras (ABR) is one of them. ABR contains pure Hingula, pure Vatsnabh, Tankan, Marich and Pipali in equal part1. The study was design to evaluate in vitro anti bacterial efficacy of Anandbhairav Ras against microorganism (pathogen) in self created bacterial food poisoning. The sample of artificial food poisoning was prepared by taking decomposed Meat Soup, Egg, Egg omelet, Milk and Potato and then after developing in vitro bacterial Food Poisoning in Artificial Stomach Media. Then the bacterial stain was taken from Artificial Food Poisoning and identifies the bacteria using gram stain method. The in vitro antimicrobial study has studied in 9 sub-groups. ABR 4 mg/well, ABR 8 mg, ABR 4 mg + Kutaj Phala twak churna 384 mg, ABR 8 mg + Kutaj Phala twak churna 768 mg, Kutaj Phala twak churna 384 mg, Kutaj Phala twak churna 768 mg, Ofloxacine 1 mg, Ofloxacine 2mg/well and Tween 80 solution. Ofloxacine 2 mg shows maximum zone of inhibition followed by kutaj 768 mg, kutaj 384 mg, ABR 4mg+ kutaj 384 mg, ABR 8mg+ kutaj 768 mg, ABR 8mg, Ofloxacine 1 mg and ABR 4mg in meat soup, egg sample, egg omelet sample, milk sample, potato sample. Hence ABR can be used in infectious disease especially by enterogenic pathogen in therapeutic dose.

Keywords: Anand Bhairav Ras, Antimicrobial Drug, Ayurveda, Enteric Pathogen, Bacteria.