PATHOPHYSIOLOGICAL CHANGES IN THE GASTRIC MUCOSA AND LIVER AFTER TREATED WITH DICLOFENAC SODIUM IN MICE

†*M. M. R. Chowdhury, †Fahmida Afrin, Md. Ali Asgar, S. S. Saha, M. N. Hoque, Md. Kamrul Islam Md. Ataur Rahman

ABSTRACT

The present study was conducted to investigate the effect of diclofenac sodium on the stomach and liver under different conditions. A total of 45 Swiss Albino mice were randomly assigned into five equal groups (n=9) (A, B, C, D & E) and were kept under 12 hour light: 12 hour dark cycle with free access to food (mice pellet @ 25gm/mice/day) and water for 40 days. Four groups were treated with diclofenac sodium @ 3 mg/kg body weight (bwt) orally in empty or full stomach with or without vitamin B12 @ 10 μg/Kg body weight through intramuscular route while one group (A) was fed only basal diet and considered as control group. Total erythrocyte count (TEC), hemoglobin (Hb) % decreased significantly (p<0.01) in the treated mice. The highest PCV value was recorded in group E on the day 40 (42.10±0.15) and the lowest value was obtained from group B at day 40 (26.41±0.11). The highest SGPT value was recorded in group B on the day 40 (42.00±0.35). Grossly, all the treated mice showed slight to moderate congestion of the gastric mucosa and in the mice having decreased TEC and Hb possesses massive hemorrhage with ulceration in the stomach. In histopathology, the lesion includes infiltration of mononuclear cells between the gastric mucosa and in the liver, central vein congestion, sinusoidal congestion, and infiltration of mononuclear cells around central vein, slightly swollen hepatocytes and fatty changes characterized by minute vacuolation.

Keywords: Stomach, Mice, Diclofenac sodium, TEC, Hb and SGPT.