REVIEW STUDY OF METHODS USED IN NGS (NEXT GENERATION SEQUENCING) IN GENE EXPRESSION

*Yusuf Talib and Talib S.H.

ABSTRACT

Study of Central Dogma in biology at molecular level is much more important which helps us to understand the genetic arrangement and aspects of gene expression at different level and scenario, which can be useful in studying the expression level for disease and their cause and precautionary measures.DNA sequencinf has come a long way since the days of two-dimensional chromatography in 1970‘s,Genome sequencing is one of the method which helps to provide the details of every sequence and Base pair, which means to determine the primary structure (sometimes falsely called primary sequence) of an unbranched biopolymer. Sequencing results in a symbolic linear depiction known as a sequence which succinctly summarizes much of the atomic-level structure of the sequenced molecule. NGS (Next generation sequencing) makes sequence-based gene expression analysis a ―digital‖ alternative to analog techniques. It lets you quantify RNA expression with the breadth of a microarray and the resolution of qPCR.

Keywords: sequencing, NGS, Microarray, Gene Expression, Sanger Method, Illumina.