GC-MS ANALYSIS OF ETHYL ACETATE EXTRACT OF SEMECARPUS ANACARDIUM NUTS

*Rajendra Singh and Arun Kakkar

ABSTRACT

In present investigation phytochemical compounds of Semecarpus anacardium nuts using GC-MS technique was carried out to determine the structure of phytochemicals present in it. The mass spectra of the phytocompounds found was matched with the National Institute of Standards and Technology library. Dried SA nut was successively extracted with petroleum ether, ethyl acetate, methanol and water using soxhlet apparatus. This extracts was purified and stored in refrigerator. The ethyl acetate extract of SA nuts was analyzed by Agilent 7890A GC with 5975MS. Nine compounds were indentified ie benzoic acid, 4-ethoxy ethyl ester, phenol. 2,4- bis (1,1-dimethyl), Eicosane, 7- hexyl. The compounds were identified by comparing their retention time and peak area with literature and structure determination was done by interpretation of mass spectra. Present investigation by GC-MS analysis of ethyl acetate extract of SA is done. Abbreviation SA = Semecarpus anacardium GC-MS = Gas Chromatography Mass Spectroscopy TLC = Thin layer Chromatography NIST =National Institute Standard and Technology TDA =Toluene-diamine EI = Electron Ionization PCI = Positive Chemical Ionization NCI = Negative Chemical Ionization RT = Retention Time MF = Molecular Formula MW = Molecular Weight amu = atomic mass unit

Keywords: Bhilwa, Phytochemical, Herbal medicine, Anacardiaceae