GCMS ANALYSIS OF BIOACTIVE CONSTITUENTS AND EVALUATION OF ANTIMICROBIAL ACTIVITY FROM THE ETHYL ACETATE EXTRACT OF NEOLENTINUS KAUFFMANII

Johnsy G* and V. Kaviyarasan

ABSTRACT

Aim: The present investigation deals with Gas chromatography (GC) Mass Spectrometry (MS) analysis of ethyl acetate extract of dried fruiting bodies of N. kauffmanii and its evaluation of antimicrobial activity. Methods: The bioactive molecules of N. kauffmanii were identified by Gas chromatographic analysis. The ethyl acetate extract of N. kauffmanii was screened for its antimicrobial property against Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa, E. coli, Klebsiella pneumonia and Candida albicans using agar well diffusion method. Results: Twenty four compounds were identified by GCMS. Major compounds are 7-methoxy-2,2-Dimethyl-2,3-Dihydro-4H-chromen-4-one (22.18%), 5-Methoxy-2,3-Diethylphenol (36.16%), Beta, Copaen-4-Alpha.OL (12.82%), 3-Dimethyl amino anisole (10.61%) etc., This is the first report on the composition of the different compounds of these fruiting bodies. The extract showed strong antibacterial activity by inhibiting the growth of both gram negative and gram positive bacteria. Conclusion: The GCMS analysis of these extract further confirm that this activity is due to the presence of antibacterial compounds present in the extract. The investigation therefore supports the traditional uses of the mushroom in the treatment of infectious diseases.

Keywords: GC-MS analysis, mushroom, antimicrobial activity, N. kauffmanii.