EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , 

 ISSN 2394- 3211

Impact Factor: 4.161

 ICV - 45.77

Abstract

METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC FOR THE SIMULTANEOUS ESTIMATION OF METFORMIN AND VILDAGLIPTINE IN PHARMACEUTICAL DOSAGE FORM

Ch. Srinivas*, Ch. Anil Kumar, B. Nagaraju and J. Nagajyothi

ABSTRACT

Validation is the process of establishing documentary evidence demonstrating that a procedure, process, or activity carried out in testing and then production maintains the desired level of compliance at all stages. In present experimentation the standard preparations were made from the API and sample preparations are from formulation. Both sample and standards are injected six homogeneous samples. Drug in the formulation was estimated by taking the standard as the reference. The average percentage assay was calculated and found to be 99.87% and 100.16 % for Metformin and Vildagliptin respectively. A simple, accurate, precise method was developed for the simultaneous estimation of the Metformin and Vildagliptin in Tablet dosage form. Retention time of Metformin and Vildagliptin were found to be 2.8min and 4.0min. % RSD of the Metformin and Vildagliptin were and found to be 0.65 and 0.9 respectively. % Recover was Obtained as 99.83% and 99.97% for Metformin and Vildagliptin respectively. LOD, LOQ values are obtained from regression equations of Metformin and Vildagliptin were 0.4ppm, 1.3ppm and 0.8ppm, 2.5ppm respectively. Regression equation of Metformin is y = 13779x + 1840, and of Vildagliptin is y = 16828x + 4143.

Keywords: Validation, API, homogeneous, simultaneous estimation, LOD, LOQ etc.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process)