DETECTION OF HELICOBACTER PYLORI IN MIDDLE EAR CLEFT AND ITS POSSIBLE ROLE IN CHRONIC SUPPURATIVE OTITIS MEDIA

Mohamed Abdel Moneim*, Hatem El habashy, Osama Refaat, Ahmed Abdel tawab and Osama Mostafa

ABSTRACT

Aim of the work: considering the close relationship between GERD and tubotympanic disorders, the presence of helicobacter pylori in middle ear cleft and its possible role in pathogenesis of CSOM has to be investigated. Patients and Methods: this prospective study was carried out at Al-Azhar University hospitals during the period from June 2014 to June 2016. forty patients were enrolled in this study, 25 (62.5%) were males and 15 (37.5%) were females. their age ranged from 18-58 years with a mean 36±2 years. the control group was consisted of 20 healthy persons with a mean age 22.6 years (range 19 - 29 years). written consent was taken from all patients and control subjects. CLO test was performed to all patients. 14C UBT was performed. Immunohistochemical staining to all specimens were performed using H. pylori antibody. Results: the CLO test was positive in 24 (60%) patients, and 14CUBT was positive in 26 (65%) patients. There was a significant positive correlation between CLO test results and 14C UBT results in the patients group (p˂ 0.002). The 14C UBT result of the patients and control were significantly different (p˂ 0.001). There were only 3 cases (7.5%) positive H. pylori by immunohistochemical study. Conclusion: The results of the present study does not support a role for H. pylori infection in pathogenesis of CSOM. Further studies are needed to confirm the presence of the organism in middle ear cleft and its role in pathogenesis of COM.

Keywords: GERD and tubotympanic, Immunohistochemical staining.