EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , 

 ISSN 2394- 3211

Impact Factor: 4.161

 ICV - 45.77

Abstract

INTRACELLULAR TOXOPLASMA GONDII ELIMINATION IN PRESENCE OF JcTI-I, A PROTEASE INHIBITOR FROM JATROPHA CURCAS SEED CAKE

Laís Pessanha de Carvalho*, Alexandra Martins dos Santos Soares, Helen Paula Silva da Costa, Ilka Maria Vasconcelos, José Tadeu Abreu de Oliveira and Edésio José Tenório de Melo

ABSTRACT

Toxoplasma gondii is the agent of toxoplasmosis, a widespread disease of medical and veterinary importance where the parasites infect all eukaryotic cells of vertebrate animals. There is no effective and low toxic treatment to eliminate the intracellular parasites, perpetuating the infection. The Jatropha species, including Jatropha curcas, acquired high economic importance as a source of high-quality biofuel and medicinal plant. Nevertheless, the anti-parasite activity of the Jatropha species has not been reported to date. In this study, the effects of a protease inhibitor from Jatropha curcas seed cake, named JcTI-I, on extra- and intracellular tachyzoites of T. gondii were assessed in vitro. Extracellular tachyzoites treated before invasion had morphological modifications but did not altered the establishment of infection. The incubation of infected host cells with 5, 10, 50, and 100 μM JcTI-I for 24 h decreased the intracellular parasite number (IC50 = 4.57 μM), without causing a significant reduction in the infected host cell population. Moreover, JcTI-I led ultrastructural alterations on intracellular tachyzoites as assessed by light and transmission electron microscopy. In summary, JcTI-I is a promising candidate for drug development to treat toxoplasmosis and other intracellular parasites.

Keywords: Physic nut; anti-Toxoplasma gondii action; serine protease inhibitor; antiprotozoal agent.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process)