FIRST ORDER DERIVATIVE METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AZITHROMYCIN AND DEXAMETHASONE FROM EYE DROPS
Ankita J. Patel*, Dharmika A. Patel and Dr. Ragin R. Shah
Simple, precise, accurate, and sensitive first order derivative spectroscopic method have been developed for the estimation of Azithromycin and Dexamethasone in market formulation. The wavelength maxima for Azithromycin and Dexamethasone are 215.16 nm and 239.07 nm respectively. For the first order derivative spectroscopic method, the zero crossing points (ZCPs) for Azithromycin and Dexamethasone were obtained at 256.63 nm and 214.35 nm respectively in 0.1N NaOH using Shimadzu 1800 UV- visible double beam spectrophotometer. The proposed method were successfully utilised for the determination of Azithromycin and Dexamethasone in eye drops, with good linearity, high percentage of recovery and acceptable precision. Different analytical validation parameters like linearity, accuracy, precision, limit of detection and limit of quantification were determined according to International Conference on Harmonization ICH Q2 (R1).
Keywords: Azithromycin, Dexamethasone, First order derivative method, UV-visible spectrophotometer.
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