QUANTITATIVE ESTIMATION AND VALIDATION OF ATENOLOL AND AMLODIPINE BESYLATE BY ABSORBANCE RATIO (Q) METHOD.
Rahul K. Godge*, Ganesh S. Shinde and Nachiket S. Dighe
The simple, rapid, accurate, precise, cost effective, and reproducible UV spectroscopic method have been
developed for the simultaneous estimation of atenolol and amlodipine besylate in bulk and combined tablet dosage
form. Atenolol and amlodipine have absorption maxima at 224 and 238.2 nm respectively. Beer’s law obeyed in
concentration range of 2‐24 μg/ml and 2‐34 μg/ml for Atenolol (ATN) and Amlodipin (AMN) respectively. The
method of Q analysis is based on measurement of absorptivity at 224 nm and at isoabsorptive point 232.2 nm. The
recovery studies from tablet are indicative of accuracy of method and are found in between 99.05‐101.16% at three
different levels of standard additions. Precision studies showed satisfactory results. A novel approach to use 0.1N
HCL as solvent is proved to be beneficial with respect to cost, stability and avoidance of organic solvent.
Keywords: Atenolol, Amlodipine Besylate, 0.1 N HCL, Q Method.
[Full Text Article]