EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , 

 ISSN 2394- 3211

Impact Factor: 4.161

 ICV - 45.77

Abstract

“DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD AND RP – HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DAPAGLIFLOZIN PROPANEDIOL AND GLIMEPIRIDE IN SYNTHETIC MIXTURE”

Ashish Patel* and Dr. Dilip maheshwari

ABSTRACT

An accurate, precise and reproducible UV-spectrophotometric methods and liquid chromatographic assay method were developed and validated for the determination of Dapagliflozin propanediol and Glimepiride in synthetic mixture. Spectrophotometric estimation was done by derivative spectroscopic method and methanol as solvent. In this method λmax for Dapagliflozin propanediol and Glimepiride were selected at 288 nm and 224nm. RP-HPLC analysis was carried out using Pearless C-18 column (4.6 x 250mm, 5μ particle size) and mobile phase composed of Acetonitrile : 10% Ortho-phosphoric acid in water pH 6.0 (70:30% v/v)at a flow rate of 1.0 ml/min and chromatogram was recorded at 228 nm. Linearity was evaluated over the concentration range of 5 -30 μg/ml and 5-30 μg/ml for Dapagliflozin propanediol and Glimepiride in UV spectrophotometric and in RP-HPLC method Linearity was evaluated over the concentration range of 1 -5 μg/ml and 1-5 μg/ml for Dapagliflozin propanediol and Glimepiride (the value of r2 = 0.9978 and r2= 0.995 found were by UV method for DAPA and GLM and the value of r2 = 0.997 and r2= 0.996 found were by RP-HPLC method for DAPA and GLM). The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values therefore the both methods can be used for routine monitoring of DAPA and GLM in the assay of Synthetic mixture of both drugs.

Keywords: .


[Full Text Article]

INDEXING

NEWS & UPDATION

  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 July 2017 Issue has been Published, Kindly check it on http://www.ejpmr.com/home/current_issues

  • EJPMR JULY ISSUE PUBLISHED

    JULY 2017 Issue has been successfully launched on 1 July 2017

  • EJPMR New Impact Factor

    Its our Pleasure to Inform you that EJPMR Impact Factor has been increased from 3.628 to 4.161 due to high quality Publication at International Level

  • Index Copernicus Value

    EJPMR Received Index Copernicus Value 45.77, due to High Quality Publication in EJPMR at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Article are invited for publication in EJPMR Coming Issue

Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process)