EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , 

 ISSN 2394- 3211

Impact Factor: 4.161

 ICV - 79.57

Abstract

PREPARATION OF SIZE CONTROLLED ALBUMIN NANOPARTICLES AND ITS IN- VITRO SAFETY EVALUATION

Neetika Taneja* and K. K. Singh

ABSTRACT

Human serum albumin has gained popularity among researchers for the fabrication of nanoparticles (NPs) due to its biocompatible, biodegradable and non-toxic nature. Particle size is one of the critical parameter for determining the in-vitro cellular uptake and in-vivo behavior of NPs. The objective of the present study was to prepare the size controlled albumin NPs and study the impact of product and process related parameter on particle size and size distribution.The NPs were prepared by the desolvation technique. Influence of various factors including ethanol rate addition, stirring speed, amount of ethanol, albumin concentration and glutaraldehyde concentration on particle size and size distribution were extensively investigated. NPs were freeze-dried using mannitol (2%) as the cryoprotectant to inhibit the particle growth during lyophilization. The particle size and polydispersity index of the optimized albumin NPs were found to be 71.09 ± 3.12 nm and -24.5mV respectively. The images from scanning electron microscope demonstrated the spherical shape of NPs. The results from the stability studies did not show any significant change in the particle size up to 4 days at 4º and 25ºC. Moreover, the developed NPs exhibited cell viability more than 85% on HT29 and HCT cells indicating the non-toxic nature of the developed NPs.

Keywords: desolvation, glutaraldehyde, cryoprotectant, lyophilization.


[Full Text Article]

INDEXING

NEWS & UPDATION

  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 November 2017 Issue has been Published, Kindly check it on http://www.ejpmr.com/home/current_issues

  • EJPMR NOVEMBER ISSUE PUBLISHED

    NOVEMBER 2017 Issue has been successfully launched on 1 November 2017

  • EJPMR New Impact Factor

    Its our Pleasure to Inform you that EJPMR Impact Factor has been increased from 3.628 to 4.161 due to high quality Publication at International Level

  • Index Copernicus Value

    EJPMR Received Index Copernicus Value 45.77, due to High Quality Publication in EJPMR at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Article are invited for publication in EJPMR Coming Issue

Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process)