EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

A SIMPLE, SELECTIVE, RAPID AND RUGGED METHOD DEVELOPMENT AND VALIDATION OF TENOFOVIR AND RILPIVIRINE IN HUMAN PLASMA USING LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRY.

B. M. S. Kumar* and B. Rajkamal

ABSTRACT

A simple, selective, rapid and rugged liquid chromatography coupled with Tandem mass spectrometric (LC-MS/MS) method was developed and validated for the quantification of Tenofovir and Rilpivirine in human plasma. The Tenofovir and Rilpivirine were eluted within 4.00 minutes using isocratic mobile phase, the column used was Zorbax 5 μ, C18, 100×4.60 mm having a mobile phase of 5 mM Ammonium acetate, 20.0% and Acetonitrile, 80% (20:80% v/v). The flow rate was 0.70 ml/min at a column temperature of 40 ± 5ºC. Analysis was performed using multiple reaction monitoring (MRM) and ionization was carried out using electrospray positive ionization mode. The retention time for Tenofovir and Rilpivirin are 0.85 min and 2.80 min respectively. The method was validated for linearity, precision, accuracy, specificity, sensitivity, matrix effect, dilution integrity, ruggedness, injection reproducibility and stability. Calibration curve range for Tenofovir and Rilpivirine were 5.000 – 600.000 and 1.000 -203.000 ng/mL respectively. The calibration curves were linear during the course of validation, with correlation coefficients ≥0.9988 and > 0.9992 for Tenofovir and Rilpivirine respectively. The precision and mean accuracy were within the acceptable limits.

Keywords: Tenofovir; Rilpivirine; LC/MS/MS; Validation.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)