EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394- 3211

Impact Factor: 4.161

 ICV - 79.57

Abstract

BIOSYNTHESIS OF SILVER NANOPARTICLES USING Saccharomyces boulardii AND STUDY THEIR BIOLOGICAL ACTIVITIES

Fatima H. Sahib*, Nawfal H. Aldujaili and Mohammed M. Alrufae

ABSTRACT

Objective: The objective of the present study is the biosynthesis of silver nanoparticles using probiotic, Saccharomyces boulardii and evaluating their biomedical activity. The biological method of nanoparticles synthesis acquires very important area due to their cost-effective and eco-friendly benefits over chemical and physical methods of synthesis. Methods: AgNPs were biosynthesized by adding silver nitrate (AgNO3) into cell free supernatant of S.boulardii at concentration (10 mM). Results: The PCR was accomplished to molecular identification of S.boulardii using specific primers for (CAG) microsatellite repeat at locus YLR177w, foreword primer (Boul 3) and reverse primer (Boul 4). Biosynthesis of AgNPs was firstly indicated by the colour alteration of reaction mixture from yellow into reddish brown. The characterization completed by XRD, SEM, EDS and AFM. The XRD presented the size of AgNPs was 19nm.The SEM was offered the shape was spherical and homogenous and the size was ranged between 63-100nm. The occurrence of elemental silver was analysed by EDS. The AFM displayed the three dimensional structure of silver nanoparticles and the average diameter was 97.44 nm. Biosynthesized AgNPs showed antibacterial activity against multidrug resistant bacteria(MDR) of both gram positive and gram negative bacteria (S.aureus, S.pyogenes, E.coli, K.pneumoniae, E.aerogens, S.typhi, A.baumannii, P.auroginosa and P.mirabilis). All tested bacterial isolates revealed their ability to form biofilm in the form of biofilm using tube and Congo red method without treated by the nanoparticles except P.mirabils, A.boumanii and E.aerogenes,but when treated with AgNPs this ability was prevented and removed in K.pneumoniae, Ps.auroginosa and C.neoformans. The AgNPs at two concentration 1mg/ml and 2mg/ml bared their antioxidant capacity in vitro by scavenging DPPH free radicles, the largest inhibition titer found in the mixture of DPPH with biogenic AgNPs and supernatant of S.boulardii at concentration 2mg/ml (87.43).

Keywords: Biosynthesis AgNPs, S.boulardii, Antibiofilm, Antimicrobial, Antioxidant Activity.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process)