ENHANCED ROS- SCAVENGING ENZYME ACTIVITY BY SALICYLIC ACID ELICITATION OF SALT TREATED SEEDS OF VIGNA RADIATA (L.) WILCZEK CULTIVARS

Shweta Dhariwal*, Laxman Nagar, Ishwar Singh and Y. Vimala

ABSTRACT

In the present investigation analyses of different concentrations of salt, i.e., NaCl (50mM,100mM and 150mM) and salicylic acid (0.01mM, 0.025mM, 0.050mM) were carried out in vitro, individually and in combinations, to find out their effect on ROS scavenging enzymes, Catalase and Peroxidase, on two cultivars of Vigna radiata -Pusa Vishal (P.V.) and SML 668. A separate set in distilled water was also analysed as control. Enzymatic activity was analysed on 3rd, 6th and 9th day of sowing after the radicle emergence. Peroxidase activity was found to be maximum in 0.01mM concentration of Salicylic Acid on 3rd day after sowing in both the cultivars. Maximum catalase activity was observed in the combination treatment of 0.025mM S.A. + 100 mM NaCl on 6th day after sowing in the cultivar Pusa Vishal. However, in case of second cultivar i.e. SML 668 maximum catalase activity was observed in the combination treatment of 0.050mM + 150mM NaCl on the 6th day after sowing. All the results indicate that use of salicylic acid led to increase in ROS scavenging enzymes and help the seeds to combat with the salt stress.

Keywords: Salicylic Acid (S.A.), Sodium Chloride (NaCl), Cultivars (c.v.), Reactive Oxygen Species (ROS).