HOMOLOGY MODELING AND STRUCTURAL STUDIES OF CELL WALL BINDING PROTEIN CHITINASE FROM NEUROSPORA CRASSA
T. V. Padmavathi, SK. Gousia, G. Srinivas and Dr. J. Naveena Lavanya Latha*
Back ground: Proteins serve a variety of functions within the cells. Some proteins are involved in structural support and movement others in enzymatic activity, and still others in interaction with the outside world. Some of the bioinformatics tools are very helpful to know the structure and functions of the proteins within the cell. Methods: The work reported here contains, the stereo chemical quality of the protein model was checked by using in silico analysis with PROCHECK and QMEAN servers. Results: In this paper the protein with 8 metal binding sites shows highest metal binding probability for the metal namely calcium in sites 1,4,5,6 & 7 with the metal probability of 0.398702, 0.627722,0.691595 0.627722 , & 0.516149 ; magnesium in sites 1,2 & 8 with the probability of 0.351869,0.571190 & 0.571190 and zinc in sites 3 & 7 with the probability of 0.643216 & 0.316166 were shown. Conclusion: The result of the study may be a guiding point for further investigations on GH18_ Chitinase protein and metal binding sites.
Keywords: cell wall proteins, chitinase, metal binding sites, homology modeling, Neurospora crassa.
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