EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , 

 ISSN 2394- 3211

Impact Factor: 4.161

 ICV - 79.57

Abstract

IN VITRO STUDY OF ANTIMICROBIAL ACTIVITY, ANTICANCER ACTIVITY, LC/MS ANALYSIS, AND SEQUENCE OF COCONUT HUSK RETTING SOIL ASSOCIATED ACTINOMYCETES IN MANDAIKADU, KANYAKUMARI DISTRICT

Sheena A. and P. A. Mary Helen*

ABSTRACT

Actinomycetes are a source of abroad variety of secondary metabolites with diverse biological activities, such as antifungal, antibiotic. Many of which have been developed for clinical use. Actinomycetes were isolated from the coconut husk retting soil sample by serial dilution agar plating method. The antimicrobial activity of actinomycetes extract was tested against pathogenic bacteria and fungi. The extract was tested against seven bacteria and five fungi. Then bioactive compounds were purified by silica gel column chromatography and it was identified by liquid chromatography-Mass spectrometry. Cytotoxic activity of actinomycetes extract on A375 cell line were analysed by MTT method. Three compounds such as 1-3-Di undecylprodiginine, Anthracene-9,10-dione, Isorenieratine were identified. The DNA of actinomycetes was isolated and the 16S rRNA gene was amplified using universal eubacteria 16S rRNA gene primers and it was phenotypically identified as Streptomyces albaduncus.

Keywords: LC.MS, 16S rRNA, coconut husk retting soil.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process)