THE EFFECT OF ANTIOXIDANT ACTIVITIES (DPPH, NO, ROS) BY BLEND ESSENTIAL OILS
Sang Nam Park, Yong Lim, Un Kyu Park, Man Kyu Huh* and Seock-Yeon Hwang*
The antioxidant activity of four blend oils from limonene oil, lavender oil, pepper mint oil, eucalyptus oil, and tea tree oil was measured by the inhibition of 1, 1- diphenyl 2-picrylhyorazyl (DPPH), nitric oxide (NO), and reactive oxygen species (ROS). All the oils showed about 90% DPPH inhibitory scavenging ability. Blending oils showed a decrease of 20% and 30% NO inhibitory scavenging ability at concentrations of 0.001 and 0.01‰, respectively. Three blending oils did not show a significant decrease of LPS-induced ROS generation. Tea tree oil and eucalyptus oil had higher activity on DPPH and NO than the corresponding other oils. Tea tree oil showed the strongest ROS activity among the oil products tested. The antioxidant activity of the samples seems to be based on their free radical scavenging capacity. The inhibitory activity was higher when the complex (blend) substance was treated than the single substance.
Keywords: antioxidant activity, 1, 1- diphenyl 2-picrylhyorazyl (DPPH), nitric oxide (NO), reactive oxygen species (ROS).
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