EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , 

 ISSN 2394- 3211

Impact Factor: 4.161

 ICV - 79.57

Abstract

STRUCTURAL CHARACTERISTICS, ANTITUMOR AND ANTI-INFLAMMATORY PROPERTIES OF POLYSACCHARIDES ISOLATED FROM THE RED ALGAE TRICLEACARPA FRAGILIS GROWING ON THE LEBANESE COAST

Zein S., Haddad M., Krivoruchko E., El-Hajje A., Hazimeh G., Kassem Z. and Kanaan H.*

ABSTRACT

Objective: The purpose of this study was to extract, to determine the functional groups and to examine the anti-proliferative and the anti-inflammatory effect of the polysaccharides sulfated galactan and carrageenan extracted from the red algae Tricleacarpa fragilis growing on the Lebanese coast. Materials and Methods: Sulfated galactan and carrageenan were analyzed using 1H NMR, and FTIR methods to reveal their structures. The polysaccharides were tested in-vitro for anti-proliferative activity against the colorectal cancer cell lines (HT 29 and HCT 116) for 24h, 48h and 72h respectively, by the MTT cell viability assay. The anti-inflammatory activity was tested on the RAW 264.7 cell line using the western blot analysis. Results: The extraction yield of sulfated galactan and carrageenan was 1.7% and 20% respectively. The structures of both polysaccharides were confirmed through the spectrum of 1H NMR. For the anti-proliferative effect, sulfated galactan was able to inhibit the growth of HT 29 and HCT 116 more than carrageenan at the different time intervals. Sulfated galactan was found to have an anti-inflammatory effect, however carrageenan induced the inflammation. Conclusion: This study discusses the structure, the functional groups, the anti-proliferative and the anti-inflammatory effect of sulfated galactan and carrageenan. Both polysaccharides were able to inhibit the growth of HT 29 and HCT 116 with sulfated galactan the more potent. For the anti-inflammatory effect, sulfated galactan inhibited the inflammation while carrageenan induced it. These effects make them candidate in the field of medicine and food industry.

Keywords: Tricleacarpa fragilis, sulfated galactan, carrageenan, anti-proliferative, anti-inflammatory.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process)