EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

TO VALIDATE STABILITY INDICATING HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS DETERMINATION OF ASSAY OF LEVOFLOXACIN AND CEFPODOXIME DRUGS IN THE PHARMACEUTICALS TABLET FORMULATIONS USING LAMOTRIGINE AS A COMMON INTERNAL STANDARD

Shaikh Javed Shaikh Afzal*, Anis Ahmed and Pathan Mohd Arif Ali Khan

ABSTRACT

Levofloxacin is a synthetic broad-spectrum antibacterial agent administered orally and intravenously. Cefpodoxime proxetil is an orally administered, extended spectrum, semi-synthetic antibiotic of the cephalosporin class. Validation of stability indicating Simple, Specific, Precise, Accurate, Linear, Rugged, Robust High Performance Liquid Chromatographic method of analysis for simultaneous determination of assay of Levofloxacin and Cefpodoxime drugs in the pharmaceuticals Tablet formulations using Lamotrigine as a common internal standard was performed. The assay was accomplished using a mixture of 0.1% Trifluoroacetic acid in Water and methanol in the volume ratio of 35:65 v/v as mobile phase on a Zorbax SB- C18, 150 mm x 4.6mm, 3.5μ as chromatographic column at a flow rate 0.700 mLmin-1 and with a uv detector at a wavelength 278 nm. The temperature of auto injector and column oven was 100C and 300C receptively. The Injection volume kept as 30 μL. linearity of the analytical method was evoluted at concentration range of 7.0041 μg/ml to 350.0350 μg/ml for Levofloxacin and 5.6048 μg/ml to 280.1050 μg/ml for Cefpodoxime respectively with Correlation coefficient (r) value more than 0.9999.The LOD and LOQ was 0.9135μg/ml and 2.7681μg/ml for Levofloxacin and 1.2387μg/ml and 3.7536μg/ml for Cefpodoxime respectively. The retention time found to be 5.01 min for Levofloxacin, 7.28 min for Cefpodoxime and 10.39 min for internal standard. Specificity, Method Precision, System Precision, Ruggedness, Robustness, Recovery, Stability of analytical solution, Filter paper selection study, Stress testing(Force Degradation) at various conditions were performed as per the ICH (Q2) recommendations. All the results were found with in acceptance criteria.

Keywords: Levofloxacin, Cefpodoxime Hydrochloride, Lamotrigine Hydrochloride, High Performance Liquid Chromatographic, Force degradation studies, Assay.


[Full Text Article]

INDEXING

NEWS & UPDATION

  • EJPMR SEPTEMBER ISSUE PUBLISHED

    SEPTEMBER 2018 Issue has been successfully launched on 1 September 2018

  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 September 2018 Issue has been Published, Kindly check it on http://www.ejpmr.com/home/current_issues

  • EJPMR New Impact Factor

    Its our Pleasure to Inform you that EJPMR Impact Factor has been increased from 4.161 to 4.897 due to high quality Publication at International Level

  • Index Copernicus Value

    EJPMR Received Index Copernicus Value 79.57, due to High Quality Publication in EJPMR at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Article are invited for publication in EJPMR Coming Issue

Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)