EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

INSULIN PROFILING AND HISTO-PANCREATIC ARCHITECTURE IN STZ–INDUCED DIABETES MELLITUS RATS’ MODEL TREATED WITH MISTLETOE AND DRUM-STICK AQUEOUS LEAF EXTRACTS

Adeeyo Olusola Atilade, *Dare Babatunde Joseph, Yusuf Uthman Ademola, Okeniran Olatayo Segun, Ebiwonjumi Adetunji Segun and Ojo Waliu Adetunji

ABSTRACT

Reports showed that antioxidants protect against disabling effects of Diabetes mellitus by mopping up free oxygen and superoxide radicals. The study is therefore aimed at investigating some ameliorative effects of Mistletoe and Drum-stick leaf extracts on STZ–induced Diabetes mellitus on the pancreatic histo-architecture and insulin profile. Fifty four (54) Wistar rats of both sexes were selected randomly into six (6) groups (G1-G6) of nine (9) animals each. The animals were acclimatized for period of two (2) weeks. Hyperglycemia was induced in twenty seven (27) overnight-fasted randomly selected rats by a single intraperitoneal administration of Streptozotocin at 70 mg/kg bw. G1 treated with 0.1 ml of phosphate buffer saline, G2 diabetic treated with Mistletoe leaf extracts only, G3 diabetic treated with Moringa leaf extracts only, G4 diabetic without treatment, G5 normoglycemic treated with Mistletoe leaf extracts only, G6 normoglycemic treated with Moringa leaf extracts only. Administration was done for six (6) weeks, animals were sacrificed by euthanasia and pancreas was harvested, fixed in 10% formol saline for H/E, Masson’s Trichrome and Von Giessen stains. A portion of pancreas was homogenized in phosphate buffer solution for estimation of pancreatic insulin concentrations using Mercodia ultra-sensitive rat insulin Elisa kit (Mercodia, Uppsala, Sweden). Mistletoe (105.22± 4.22 mg/dl) and Drum-stick (94.33± 5.62 mg/dl) significantly lowered blood glucose levels, increase in body weight and relative pancreatic weight were observed relative to the untreated diabetic rats. The Pancreatic Insulin levels of Mistletoe (3.4±0.186 μg/L) and Drum-stick (3.5±0.088 μg/L) treated hyperglycemic groups were not significantly different from normal control (3.8±0.25 μg/L) P>0.05. The pancreatic histo-architecture was maintained in the pancreatic islet of Drum-stick treated group with improved vascularization, increase in β cell mass. Elastic fibers were extensively deposited in the acini of untreated diabetic group. Thus aqueous leaf extracts of Mistletoe and Moringa maintained pancreatic islet morphology and insulin profiling.

Keywords: hyperglycemia, Anti-diabetics, Antioxidants, Leaf Extracts, Pancreas and Wistar rats.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process)