EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

ISSR MARKERS FOR COMPARATIVE ANALYSIS OF GENETIC VARIABILITY IN SOLANUM L. SPECIES OF SOUTHERN WESTERN GHATS OF KERALA, SOUTH INDIA

Anil Kumar V. S.*, Sunila A. V., Remya Krishnan and Murugan K.

ABSTRACT

Aims: To analyze the genetic variability among 17 Solanum L. taxa from the Southern Western Ghats of Kerala along with common cultivars S. melongena Neelima and S. tuberosum by employing inter simple sequence repeats. Study design: using15 ISSR primers, the purified DNA from the plant samples were tested for their variability. Methodology: The DNA samples were isolated and purified from various plant materials. PCR amplification was done. ISSR primers were used for hybridization and the scorable bands were analyzed. This was compared for the presence of shared and unique bands and finally the percentage of genetic distance calculated and ISSR dendrogram was constructed. The grouping of the clusters denotes their genetic relatedness. Results: 14 ISSR primers showed polymorphism and the highest number of polynmorphic amplification fragments was 22, obtained with the primer number 02. The least number of bands obtained with the primers 05 and 03. Conclusion: The genetic variability existing among various Solanum species could be effectively demonstrated using ISSR analysis.

Keywords: Dendrogram, Inter simple Sequence Repeats, Polymerase Chain Reaction, Solanum.


[Full Text Article]

INDEXING

NEWS & UPDATION

  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 May 2018 Issue has been Published, Kindly check it on http://www.ejpmr.com/home/current_issues

  • EJPMR MAY ISSUE PUBLISHED

    MAY 2018 Issue has been successfully launched on 1 May 2018

  • EJPMR New Impact Factor

    Its our Pleasure to Inform you that EJPMR Impact Factor has been increased from 4.161 to 4.897 due to high quality Publication at International Level

  • Index Copernicus Value

    EJPMR Received Index Copernicus Value 79.57, due to High Quality Publication in EJPMR at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Article are invited for publication in EJPMR Coming Issue

Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process)