EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

DIAGNOSIS OF SALMONELLA ASSOCIATED WITH DIARRHEA IN BOVINE CALVES AND ROLE OF LACTOFERRIN IN TREATMENT

Baker N. M., Noha A. Beder, Mahmoud A. A., Hamouda A. H. and *Ismail I. M.

ABSTRACT

Clinically, bacteriologically, serologically and by polymerase chain reaction (PCR); two hundred and fifty calves, with an age of 1 day to 2 months old were examined to diagnosed and identify salmonella and its virulence factors. Our study showed that the clinical signs ranged from yellowish watery to greenish watery diarrhea with blood clots and/or mucus. The mucous membranes ranged from normal, congested or pale. The eyes of affected animals were sunken in most cases due to dehydration while the bacteriological isolation revealed that 35(14%) calves were positive to salmonella including 57.14% cow calves and 42.85% buffalo calves. Serotyping of salmonella isolates revealed that S. enteritidis was 17.14%, S. typhimurium was 45.7%, S. infantis was 14.28%, S. tsevie was 14.28 and S. magherafelt was 8.57%. PCR carried out on 15 isolates; invA gene was positive in 13 cases, stn gene was positive in 6 cases and pef gene was only in 2 cases. The antimicrobial sensitivity indicated that the most effective antibiotics were observed for Cefquinome (85.7%), Cefepime (77.1%), Ceftriaxone (60%) and Ciprofloxacin (57.1%). After using of Lactoferrin the total anti- oxidant capacity increased while H2O2 decreased and the cure rate of salmonella infected animals increased as the number of dead animals decreased from 6 (17.14%) to one (2.8%), so we concluded that the diarrhea of salmonella affected animals was ranged from yellowish watery to greenish watery diarrhea with blood clots and mucus, the most prevalent serotypes of salmonella were S. Typhimurium and S. Enteritidis and the treatment with antibiotics in addition to lactoferrin increased the cure rate and decrease the recurrency of the disease.

Keywords: Salmonellosis in bovine calves, serological identification of Salmonella, PCR, lactoferrin.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process)