PRELIMINARY PHYTOCHEMICAL SCREENING, IN VITRO THROMBOLYTIC AND MEMBRANE STABILIZING ACTIVITIES OF GYNURA NEPALENSIS LEAVES OF BANGLADESHI ORIGIN

Razia Sultana* and Sitesh Chandra Bachar

ABSTRACT

Gynura nepalensis (Family: Asteraceae), is one of the commonly used medicinal plants that was investigated for in vitro thrombolytic and membrane stabilizing activity. People of Bangladesh have been using this herbaceous plant for various medicinal purposes from the treatment of diabetes mellitus to control of blood pressure etc. Preliminary Phytochemical screening influenced the presence of different phytoconstituents e.g. alkaloids, glycosides, tannins, carbohydrates, phenols, resins and coumarins. The crude ethanolic extract of the leaves of G. nepalensis and its different partitionates were studied by using Streptokinase (SK) and acetyl salicylic acid (ASA) as standards for thrombolytic and membrane stabilizing activities. In case of in vitro thrombolytic activity, the ethanolic fraction of leaves (EF) Showed 50.84 ± 0.79 %, n-Hexane fraction (HXF) 47.70± 0.43 %, chloroform soluble fraction (CF) 42.94± 0.13%, aqueous soluble fraction (AQF) 41.15± 0.92% and pet-Ether soluble fraction (PF) 24.98 ± 0.35% against thrombosis whereas standard SK showed 64.19 ± 0.22%. ASA exhibited 71.25 ± 0.21% haemolysis inhibition of human red blood cell (RBC) membrane in normal condition whereas EF inhibited 64.79 ± 0.51%, HXF inhibited 58.63 ±0.36%, PF inhibited 51.98 ±0.12%, CF inhibited 47.42 ±0.22%, and AQF 44.51 ±0.10%. In heat induced condition, the EF inhibited 54.95±0.42%, HXF inhibited 50.57±0.79%, CF inhibited 44.92±0.24%, PF inhibited 48.90±0.71%, and AQF 39.93±0.46% respectively and ASA showed 61.64±0.31% for against unstable membrane. The abovementioned results suggested that the ethanolic extracts exhibit favourable influence for the use of this plant as traditional medicine and its further study.

Keywords: Gynura nepalensis, Asteraceae, thrombolytic, membrane stabilizing activity.