HPTLC-DENSITOMETRIC ANALYSIS OF ALPRAZOLAM AND MELATONIN IN THEIR COMBINED TABLET DOSAGE FORM
Dr. D. Umamaheshwari*, Dr. M. V. Kumudhavalli, Dr. S. Alexandar, Dr. M. Kumar and C. Prakash
The present study describes development and subsequent validation of stability indicating HPTLC methods for simultaneous estimation of Alprazolam (ALP) and Melatonin (MELA) in their combined formulation. The proposed HPTLC, separations were performed on silica gel 60 F254 using Ethyl acetate: chloroform: hexane: glacial acetic acid (6 : 3 : 4 : 0.7 v/v) as mobile phase. The compact bands of ALP and MELA at ð‘…ð‘“ 0.06 and 0.19 respectively were scanned at 266 nm. Linear regression analysis revealed linearity in the range of 50 to 300 ng/band for ALP and 300 to 1800 ng/band for MELA respectively. For both the methods, dosage form was exposed to thermal, photolytic, acid, alkali and oxidative stress. The methods distinctly separated the drugs and degradation products even in actual samples. In conclusion, the proposed HPTLC methods were appropriate for routine quantification of ALP and MELA in tablet formulation.
Keywords: The present study MELA in tablet formulation.
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