EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC FOR ASSAY OF TRAMETINIB IN PHARMACEUTICAL DOSAGE FORM

B. Ramakrishna, N. Sarat Babu and NVS Naidu*

ABSTRACT

A simple, selective, precise and accurate High Performance Liquid Chromatographic method for the analysis of Trametinib was developed in Pharmaceutical Dosage form. Trametinib showed maximum absorption at 245nm. This method achieved by using isocratic elution with ratio ammonium acetate buffer: methanol: acetonitrile: tetrahyrofuron mobile phase at 40:30:20:10V/V. The developed HPLC method was validated for Linearity, Accuracy, Interday Precision, Specificity & Selectivity, Robustness, Solution stability, limit of detection & limit of quantification. Stability Indicating Methods were developed for Trametinib in Pharmaceutical Dosage form under hydrolytic stress condition (5N HCl, 5N NaOH); Oxidation condition (5% H2O2) and dry heat condition, Thermal condition, UV light. From this study, it was found that drug is not susceptible for degradation to hydrolytic condition, oxidation condition, dry heat, UV light, and thermal condition. All the developed methods were successfully applied to determine the drug in Pharmaceutical preparation.

Keywords: HPLC, Trametinib, Spectrophotometric method, Pharmaceutical Dosage.


[Full Text Article]

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  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 December 2018 Issue has been Published, Kindly check it on http://www.ejpmr.com/home/current_issues

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)