ISOLATION OF OCTADECANEDIOIC ACID FROM CASSIA FISTULA LINN AND ITS BIOLOGICAL EVALUATION
Brinda Bolar and *Gangadhar A. Meshram
Cassia fistula L., a semi wild Indian Labernum also known as the golden shower tree is an ornamental tree with beautiful bunches of yellow flower. It is highly reputed for its laxative and purgative properties. The extracts of cassia fistula are widely used as traditional antidiabetic medicines. One of the theraupatic approach for preventing diabetes mellitus-2 is to retard the glucose absorption in the blood via inhibition of glucosidase viz glucoamylase and α-amylase. In the present study Octadecanedioic acid was isolated from the bark of Cassia fistula. The structure was elucidated on the basis of MS, NMR and IR. Octadecanedioic acid exhibited potent inhibitory activity against glucoamylase and α-amylase with IC50 value 56μg/mL and 48μg/mL respectively. The antioxidant activity was evaluated by DPPH method. The results indicated that Octadecanedioic acid possess considerable antioxidant activity. The highest radical activity was detected at 81μg/mL. As a result Octadecanedioic acid was isolated from Cassia fistula can be used for antioxidant therapy during management of Diabetes Mellitus Type -2.
Keywords: Cassia fistula, Octadecanedioic acid, glucoamylase, ?-amylase, antioxidant activity.
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