EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF METAL COMPLEXES DERIVED FROM 2-(PHENYLAMINO)ACETOHYDRAZIDE LIGAND

Fathy A. El-Saied*, Tarek A. Salem, Bishoy Y. El-Aarag, Manar A. Hashim

ABSTRACT

Metal complexes of the ligand, 2-(phenylamino)acetohydrazide (HL) with Cu(II), Ni(II), Co(II), Mn(II), Zn(II), Cr(III) and Fe(III) ions were synthesized and characterized using elemental, spectral (IR, UV- VIS), and thermal analyses (TGA) as well as magnetic moment and molar conductance measurements. The ligand in the obtained solid complexes coordinates as a bi-dentate either as a neutral via the amino group nitrogen and carbonyl oxygen atoms or anilino NH or as a monobasic via the amino group nitrogen atom and enolic (C-O) oxygen atom in the enolimine-tautomeric form. The cytotoxic effect of these complexes was estimated against Hep-G2 cancer cell lines. Also, the in-vivo model of hepatic fibrosis was induced by the intraperitoneal (i.p) injection of carbon tetrachloride (CCl4) in mice and the possible antifibrotic activities of these complexes were estimated. Moreover, liver function, histopathological examination of liver, and expression of caspase 3 in liver tissues were assayed. Results revealed that all the newly synthesized complexes exhibited cytotoxic effect on Hep-G2 cell lines. Also, administration of Cu(II) complexes to the CCl4-intoxicated mice led to improvement the hepatic function and liver histology. Furthermore, the treatment of CCl4-intoxicated with ligand and copper complexes led to a marked decrease in the caspase 3 expression in nucleus and cytoplasm of hepatic cells. In conclusion, Cu(II) complexes of hydrazide derivatives, specially Cu(II) perchlorate, showed anticancer and antifibrotic activities higher than the ligand. The potential activity of the tested complexes might be due to their cytotoxicity and ability to reduce the expression of apoptotic gene caspase-3 that helps in the repairing of liver cells.

Keywords: Cytotoxic activity; Hep-G2; Fibrosis; Caspase-3; Hydrazide ligand; Metal complexes.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)