EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

IN VIVO EXPERIMENTAL STUDIES ON CLOSTRIDIUM PERFRINGENS Tpel TOXIN

Mostafa Galal, Ola Basha, Hoda Abd-Ellatieff, Abdelrahman Abou Rawash and Madiha Salah Ibrahim*

ABSTRACT

The present study was conducted to evaluate the prevalence of C. Perfringens in boiler chickens in El Behiera and Matrouh governorates. Samples (n=362) were collected from intestine (n=162), feed (n=100) and litter (n=100). The prevalence of C. Perfringens was 49.1, 82.5, 60 and 50% in apparently healthy birds, diseased birds, feed and litter, respectively. Isolates were confirmed by Matrix-assisted laser desorption/ionization (MALDI). Antimicrobial susceptibility of C. perfringens showed sensitivity to Cefotaxime, Chloramphenicol, Bacitracin, Norfloxacin, Ciprofloxacin, Doxycycline, Telmicosin, Amoxicillin + Clavulanic acid and Clindamycin, while resistant to Gentamycin, Erythromycin, Amoxicillin and Neomycin. Eighteen C. perfringens isolates were examined by multiplex PCR using specific primers for Alpha, Beta, Epsilon and Iota genes. The alpha gene was detected in 17 of the examined isolates. By PCR, only five isolates were positive for the Tpel toxin gene. Further, the Net-B toxin gene was detected in one isolate. By neutralization test in mice and Dermonecrotic test in Albino guinea pigs, 5 C. perfringens isolates were toxigenic (type-A). Furthermore, experimental effect of trypsin on Tpel toxin in guinea pigs showed inhibition of the Tpel pathogenic effect. Bla and Erm-B genes specific to resistance of C. perfringens to beta lactam and macrolides, respectively, were detected by PCR in 100% and 60% of the tested isolates, respectively. These results showed matching between phenotypic and genotypic resistance in C. Perfringens. Further, environmental factors (feed and litter) represent an important source of toxigenic C. perfringens infection and apparently healthy birds may act as a major source of infection

Keywords: Broiler chickens, C. Perfringens, Tpel gene, PCR, Trypsin, Beta lactams, Macrolides.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)