EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

DETECTION OF SALMONELLA SEROVARS AND THEIR COMMON VIRULENCE GENES FROM DIFFERENT CHICKEN BREEDING SYSTEMS

Wael Said El Shafey, Mohammed Farouq Hashim, Ahmed Abo-Elmagd Bekheet and Madiha Salah Ibrahim*

ABSTRACT

The present study investigated the occurrence, antibiotic sensitivity patterns, and virulence gene profiling of Salmonella serovars in chicken farms in Behaira governorate, Egypt. Three hundred samples including chicken's internal organs, cloacal swabs, litter samples and eggs were examined for the presence of Salmonella by standard microbiological techniques. All Salmonella isolates were tested for their sensitivity against 11 antibiotics and subjected to virulence genotyping by polymerase chain reaction (PCR). The overall isolation percentage of Salmonella was 13%. The prevalence of Salmonella was 6.8% in broilers, 42.3% in layers, 0% in breeders and cloacal swabs, 6.5% in litter and 15.6% in eggs. Four different serovars were found, with the predominant one being Salmonella enteritidis. Salmonella isolates were sensitive to most of the tested antibiotics, but they exhibited absolute resistance against ampicillin and cefotaxime. PCR investigation of invA, mgtC, sopB and bcfC virulence genes in 8 Salmonella isolates revealed that all tested genes were expressed in all examined isolates. These results would greatly help in understanding the prevalence of virulence genes and antibiotic sensitivity among Salmonella serovars in chicken.

Keywords: PCR, Salmonella, Serotypes, Virulence Genes.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)