LUPEOL AND EUGENOL ENRICHED FRACTION FROM PIPER BETEL LEAF OIL AND THEIR QUANTITATIVE ESTIMATION BY HPTLC FOR QUALITY CONTROL PURPOSES
Basudhrity Banerjee, Lokesh K. Bhardwaj*, Nitin Agrawal, H. Lalhlenmawia, Siddhartha Datta, Avijit Bhowal and Subhash C. Mandal
Eugenol and lupeol are reported from the leaf oil of Piper betle Linn.(Piperaceae). Both the compounds have been shown to give interesting biological activities and hence serve as biomarkers. We report extraction of Piper betle leaf oil, fractionation and identification by a simple TLC densitometric method for the quantification of eugenol and lupeol in Piper betle leaf oil. The contents of eugenol and lupeol in the samples of Piper betle leaf oil, as estimated by the proposed method, were found to be 8.321 Î¼g and 634.94 n.g respectively. The proposed HPTLC method for the estimation lupeol and eugenol was found to be simple, precise, specific, sensitive and accurate and can be used for routine quality control of Piper betle leaf oil.
Keywords: Thin layer chromatography, Eugenol and lupeol, Medicinal plants, Piper betle.
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