DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR ESTIMATION OF MOXONIDINE IN BULK AND FORMULATION
Tejavath Venu*, Dr. M. Dhanalakshmi and Md. Asra Farheen
A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the
validated of Moxonidine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a
Phenomenex Luna C18 (4.6 x 250mm, 5μm) column using a mixture of Methanol and Water (75:25% v/v) as the
mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 223nm. The retention time of the
Moxonidine was 2.7 ±0.02min. The method produce linear responses in the concentration range of 20-100ppm of
Moxonidine. The method precision for the determination of assay was below 2.0% RSD. The method is useful in
the quality control of bulk and pharmaceutical formulations.
Keywords: Moxonidine, RP-HPLC, validation.
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