IMPLEMENTATION OF EX- OVO CHICK EMBRYO DEVELOPMENT MODEL FOR EVALUATION OF ANGIOGENESIS AND VASCULOGENESIS STUDIES

Aruna Kanase*, Shilpa Pachapurkar, Anuya Mane and Jaywant Jadhav

ABSTRACT

The need of continuous monitoring throughout the experimental period initiated authors to test the ex-ovo embryo development model for CAM angiogenesis to test the drugs. The experimental design involved comparison of traditional in-ovo model (using window technique) with experimental ex-ovo shell less culture. HBSS which is conventionally used as medium for test drugs/chemicals was introduced at 48, 72, 96 and 120 hrs of development and embryos were studied at 144hrs when CAM development is complete. In data analysis primary, secondary and tertiary (capillary networking) vitelline veins were numerically compared. The data shows ex-ovo shell less culture is of equal competence of traditional in-ovo window method with an advantage of intermediate monitoring when drugs are used.

Keywords: Angiogenesis, vasculogenesis, CAM, ex-ovo model, in-ovo model, window method.