SYNERGISTIC ANHELIMENTIC ACTIVITY OF DIFFERENT COMPOSITION OF VOLATILE OIL OF EUCALYPTUS AND NEEM OIL BY PHERETIMA POSTHUMA
Amrita Asthana*, Somendra Kumar Maurya, Satya Prakash Maurya, Anita Maurya and Pooja Maurya
The research work deals with the screening of synergistic anthelmintic activity of different composition of
Eucalyptus (Eucalyptus globulus) and Neem (Azadirachta indica) oils. The Pheretima posthuma were divided into
9 groups and were administered orally in different ratios of Eucalyptus and Neem 1:1, 1:2, 1:3, 2:1 and 3:1, Control
(normal saline DMSO and Tween 80) & Standard (Albendazol) for 5 hrs and to investigate the anti-helmintic
activity of eucalyptus and neem oils may used in 1:1, 1:2, 1:3, 2:1 and 3:1 ratios and dissolved in DMSO and
Tween80 were investigated for activity on Indian earthworms (Pheretima posthuma). Both ratios show a dose
dependant reduction in paralysis and death time of the worm, were found to be more effective. Results were
expressed as mean ± SE. ANOVA followed Dunnet‟s multiple “t” test. P values < 0.05 are considered statistically
significant using software Graph Pad Prism 5 and found to produce paralysis than death time recorded in
experimental animals and the efficacy of the different combination of oil was found to be comparable with that of
standard drugs used Albendazol.
Keywords: Anthelmintic activity, DMSO, Tween 80, Albendazol, Pheretima posthuma.
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