EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

DNA CONTENT IN DRUPE FRUITS WERE SIGNIFICANTLY MORE THAN THAT IN POME, HESPIRIDIUM, PEPO AND COMPLEX FRUITS BECAUSE OF MORE FLESH IN DRUPE

Nishat Chowdhury*, Shingo Ueno and Naznin Parbin

ABSTRACT

Genomic DNA extraction is an important aspect of plant molecular biological research. The objective of the study was to recommend the cheap and efficient genomic DNA extraction method for some economically important fruit species of Bangladesh. The modified plant genomic DNA extraction methods explained by drupe, pome, hespiridium, pepo and complex fruits extraction kit (Qiagen) method were applied with nine different fruit species such as Carica papaya (Papaya), Musa aouminata (Banana), Scolytus unipinosus (Grape), Psidium guajava (Guava), Malus angustifolia (Apple), Citrus aurantiam (Orange), Manilk arazapota (Whitelead), Pyrus commonis (Pear), Anaras conassus (Pineapple).Based on the quantity of the extracted genomic DNA tested by measuring the absorbance at 260 nm using Nanodrop® ND-1000 spectrophotometer, quality determined by the ratio of A260 / A280 and the amplifiable quality of DNA determined by the horizontal agarose gel electrophoresis using 1% agarose in TBE buffer at constant voltage of 60V, the method explained by Cheng et al and the Genomic DNA extraction kit yielded good quality DNA with satisfactory concentration for all the fruit species tested. Therefore the modified method of Cheng et al, 1987 could be recommended for the efficient and cost effective DNA extraction from fruit species instead of the commercially available expensive and chemically hazardous DNA easy plant kit method. The DNA content in drupe fruits were significantly more that that in pome, hespiridium, pepo and complex fruits (two tailed t-test value 0.26).

Keywords: .


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)