EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

CHARACTERIZATION OF A CLINICAL ISOLATE OF STAPHYLOCOCCUS AUREUS, AND THE ACTION OF LINEZOLID ON GROWTH PROPERTIES AND TOXIN PRODUCTION

Sylvanus Akpak Upula* and Kanayo Eugene Ikeh

ABSTRACT

Staphylococcus aureus emergence as a significant pathogen has been enhanced by its increased resistance to many antibiotics due to its ability to express several virulence factors, and extracellular toxins, as seen in Methicillinresistant S. aureus (MRSA) strains. This study, investigated an S. aureus clinical isolate obtained from Glasgow Royal infirmary MRSA reference laboratory, using phenotypic and molecular methods in assessing its susceptibility to linezolid, ability to produce biofilms, known toxins, and the impact of treatment with sublethal linezolid concentration on toxin(s) expression. Result revealed Minimum Inhibitory Concentration (MIC) of linezolid on S. aureus planktonic cells as 4mg/L, Minimum Bactericidal Concentration (MBC) as 32mg/L, and MBC/MIC ratio of 8. Antibiotic time kill assay revealed linezolid effect on the planktonic cells of the S. aureus isolate as bacteriostatic; as viable count reduction was approximately 2log10. Biomass measurement of the S. aureus isolate by comparison with RP62a; a known biofilm-producing strain, indicated that it formed strong biofilm, in biofilm, linezolid concentration at 10×MIC had no significant effect (p>0.01) on its viability whereas, at 40×MIC linezolid effect was significantly greater (p<0.01), but unable to eliminate its viability. The S. aureus clinical isolate was shown to produce Staphylococcal Enterotoxin A and toxic shock toxins, and when challenged with sublethal linezolid concentration (0.25×MIC), its expression of both toxins proteins was downregulated by 2 folds. This study suggests linezolid ability to limit expression of vital S. aureus virulence factors and reinforces linezolid for consideration, in the treatment of severe S. aureus infections.

Keywords: Staphylococcus aureus, MRSA, Linezolid, Planktonic, Biofilms, Toxins.


[Full Text Article]

INDEXING

NEWS & UPDATION

  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 January 2019 Issue has been Published, Kindly check it on http://www.ejpmr.com/home/current_issues

  • EJPMR JANUARY ISSUE PUBLISHED

    JANUARY 2019 Issue has been successfully launched on 1 January 2019

  • EJPMR New Impact Factor

    Its our Pleasure to Inform you that EJPMR Impact Factor has been increased from 4.161 to 4.897 due to high quality Publication at International Level

  • Index Copernicus Value

    EJPMR Received Index Copernicus Value 79.57, due to High Quality Publication in EJPMR at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Article are invited for publication in EJPMR Coming Issue

Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)