ANALYSIS OF ORAL MICROORGANISMS BEFORE AND AFTER PERIODONTAL TREATMENT USING REAL-TIME PCR
Yun-Kyung Kim* and Gey-Pyo Kim
It is important to analyze oral microorganisms in patients with periodontal disease because this disease is caused by
the proliferation of specific types and numbers of microorganisms in the oral cavity. The aim of this study was to
analyze the changes in the number and type of pathogenic microorganisms in the oral cavity using real-time PCR
before or after periodontal treatment in patients aged 50 years or more who visited the D dental clinic in Busan.
The levels of pathogenic microorganisms before and after periodontal treatment were 12.44 ± 1.76% and 7.01 ±
0.10%, respectively. Thus, the level after periodontal treatment was significantly decreased compared to before
treatment. Analysis of the major pathogens of dental caries revealed that Sm, Ss, and major pathogens of
periodontal disease, including Ac, Pg, Tf, Td, Pi, Fn, Pm, Cr, Pn, and En, were significantly decreased compared to
before periodontal treatment. Quantitative analysis of periodontal pathogens in the clinical by real-time PCR
demonstrated the effectiveness of the treatment, which will help to improve the oral hygiene management ability of
Keywords: Dental clinic, Oral microorganisms, Periodontal disease, Periodontal treatment, Real-time PCR.
[Full Text Article]