EXTRACTION, PHYTOCHEMICAL EVALUATION AND CHARACTERIZATION OF POLYPHENOLS AND FLAVONOIDS FROM AQUEOUS METHANOLIC LEAF EXTRACTS OF INDIGENOUS PLANT CLERODENDRUM COLEBROOKIANUM WALP AND CENTELLA ASIATICA LINN OF NORTH EAST INDIA
Ratna Jyoti Das*, Daphisha Marbaniang and Bhaskar Mazumder
Extraction of polyphenols and flavanoids were done for two indigenous plants Clerodendrum colebrookianum walp and Centella asiatica. Extraction was done by both cold maceration and continuous hot percolation. Determination of total phenolic and flavanoid content was carried out. Characterizations was done by TLC study, UV analysis, melting point determination and FTIR study. More total phenolic content found in Clerodendrum colebrookianum walp (621.02 ± 0.03 mg of GAE/gm plant extract) and total flavonoid content was found more in Centella asiatica L.(596.40 ± 0.53 mg of Quercetin equivalent/gm plant extract). UV and FTIR study revealed the presence of both flavonoids and phenolics in the respective extracts as compared with gallic acid and quercetin. High content of phenolic and flavanoid shows the sign of antioxidant activity of the respective plants. Both the plants showing rich in total polyphenols and total flavonoids. Datas were mean ± SD and analyzed by One-way ANOVA, using Graphpad INSTAT. Confidence interval has been considered as 99% and p< 0.01 were considered significant. Phenolics have shown to possess an important antioxidant activity toward free radicals, which is principally based on the redox properties of their phenolic hydroxyl groups and the structural relationships between different parts of their chemical structure. It has been established a highly positive relationship between total phenols and antioxidant activity in many plant species.
Keywords: Polyphenols, flavonoids, extraction, indigenous plant, phytochemical evaluation.
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