EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

EFFECT OF WHOLE CELL AND OUTER MEMBRANE PROTEIN VACCINES ALONG WITH IMMUNOADJUVANT ANDROGRAPHIS PANICULATA AGAINST STAPHYLOCOCCOSIS DISEASE IN FINGERLINGS OF LABEO ROHITA

Deepalakshmi R.* and Brindha Devi G. B.

ABSTRACT

Whole cell and Outer membrane protein vaccines, when administered with immunoadjuvant increases the efficacy of the vaccine uptake and boosts the innate immune system of fish. The present study is to develop the whole cell and outer membrane protein vaccine, administered along with immunoadjuvant Andrographis paniculata in Labeo rohita fingerlings, post challenged with virulent Staphylococcus aureus. 25 fingerlings were pre-treated with hyper- osmotic solution of 2% NaCl and vaccinated twice with booster dose on day 1 and second dose on day 30. After 30 and 60 dpv (days post vaccination) fingerlings were bath challenged for up-to 1 hour in the lethal dose of 1x108 cells/ml. SDS-PAGE results revealed, WC vaccine with band length of 27.95 KDa and OMP vaccine with band length of 27.43-35.43 KDa respectively. Total protein concentration of WC vaccine was found to be 34μg/ml and 22μg/ml in OMP vaccine respectively. After 30 and 60 dpv there was significant increase in RPS rate of experimental groups, showing a RPS 84% in OMP and 92% in OMP+A when compared to a RPS of 76% in WC and 80% in WC+A respectively. The results inferred that, both OMP and WC vaccine along with an immunoadjuvant could be effective vaccine candidates against staphylococcosis disease in Labeo rohita fingerlings.

Keywords: Labeo rohita, Whole cell, Outer membrane protein, Staphylococcus aureus, immunoadjuvant, Andrographis paniculata, mortality, relative percent survival.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)