PHYTOCHEMICAL STUDY AND EVALUATION OF ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF VITEX DONIANA SWEET (VERBENACEAE)
Ouattara Karamoko, Ouattara Lacinan*, Ouattara Abou, Rajib Majumder, Debopam Banerjee, Palashpriya Das and Suman Khowala
Owing to difficulties encountered in the use of synthetic antioxidants, research is moving more and more towards natural antioxidants particularly those from plants. It is in this context that we were interested Vitex doniana Sweet, a plant commonly used in the Ivorian pharmacopoeia for the treatment of several diseases. After an experimental study on a phytochemical screening of the methanolic extract of the plant, a quantitative estimate of total polyphenols (by the Folin-Ciocalteu method) and total flavonoids (by the method aluminum trichloride) was performed. Then; the antioxidant activity of the extract was evaluated through three methods: the method of DPPH (2.2 diphenyl picrylhydrazyl 1), the FTC (ferric thiocyanate) method and TBARS (thiobarbituric acid) method. The results obtained are shown the presence of various compounds including polyphenols groups. Furthermore, inhibition of DPPH radical coupled to the inhibition of lipid peroxidation by test FTC and the TBARS showed significant antioxidant activity for this extracted with a percent inhibition of 53% recorded by FTC and 52% by TBARS. The antioxidant activity of the observed plant would be partly due to its abundance of polyphenols.
Keywords: Vitex doniana, methanolic extract, phytochemical screening, antioxidant activity, Côte d’Ivoire.
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