EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

AN APPROACH TO DIAGNOSIS OF T-CELL AND NK-CELL NON - HODGKIN LYMPHOMAS BY FLOW CYTOMETRIC IMMUNOPHENOTYPING ON FINE NEEDLE ASPIRATE OF LYMPH NODE

Dr. Khondoker Hafiza Khanom, Dr. Shirin Tarafder* and Professor Humayun Sattar

ABSTRACT

Introduction: Lymphoid Neoplasms are defined as clonal tumors of mature and immature B cells, T cells or natural killer (NK) cells at various stages of differentiation. Because NK cells are closely related and share some immunophenotypic and functional properties with T cells, these two classes of neoplasms considered together. Distinct T-cell/NK-cell NHL subtypes have unique characteristics and often warrant individualized diagnostic and therapeutic treatment strategies. Objective: The aim of this study was to diagnose T-cell/NK-cell Non-Hodgkin lymphoma by Flow cytometric immunophenotyping (FCI) on fine needle aspirate (FNA) of lymphnode following immunophenotypic diagnostic criteria based on expression of CD markers. Method: All samples were preliminary assessed by fine needle aspiration cytology (FNAC) as NHL or lymphoproliferative disorder (LPD). FCI was performed with a complete panel of antibodies (CD3, CD4, CD8, CD5, CD7, CD10, CD19, CD20, CD23, CD22, CD25, CD30, CD45, CD79a, CD79b, CD95, CD56, FMC7, CD40, CD15, Kappa, Lambda and Bcl-2) by dual color flow cytometry. FCI data were interpreted to diagnose and subclassify NHL according to WHO classification. Wherever possible the diagnoses were compared with available immunohistochemistry (IHC) histopathology reports. Result: During one year period (from March 2016 to February 2017) 31 cases of Non-Hodgkin lymphoma were diagnosed by FCI of which 13 (41.9%) cases were T-cell type of Non-Hodgkin lymphoma and 2 (6.5%) cases were Aggressive Natural killer cell leukemia. Among T-cell type NHL, 3(23.1%) cases were Peripheral T-cell lymphoma (NOS), 3(23.1%) cases were T-lymphoblastic lymphoma, 4(30.8%) cases were Angio-immunoblastic T-cell lymphoma, 2(15.3%) cases were Anaplastic large cell lymphoma, and 1(7.7%) case was Adult T- cell lymphoma. CD45 and both membrane and cytoplasmic CD3 were positive in all types of T-cell NHL except T-lymphoblastic lymphoma, in which membrane CD3 negative but cytoplasmic CD3 was positive. There were variation in CD4, CD8, CD5, CD7, CD10, and CD30 markers. In case of Aggressive NK-cell NHL, CD56 and BCL2 was found to be positive. Identification by FCI is 23.4% higher in T-cell lymphoma than IHC and is 27.8% higher than histopathology. No NK-cell type of Non-Hodgkin lymphoma was detected by histopathology. Conclusion: By histopathology, it is almost impossible to detect all subtypes specially T-cell and NK-cell type of Non-Hodgkin lymphoma but flow cytometric immunophenotyping can directly recognize the different subtypes associated with disease progression by detecting specific CD markers.

Keywords: Flow cytometry, Immunophenotyping, T-cell/NK-cell Non-Hodgkin lymphomas, Fine needle aspirate.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)