EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

INCIDENCE AND DETECTION OF METALLO-BETA-LACTAMASE PRODUCING KLEBSIELLA PNEUMONIAE IN A TERTIARY CARE HOSPITAL

Dr. Atul Khajuria* and Ashok K. Praharaj, MD

ABSTRACT

Objective: To examine the distribution, emergence and spread of genes encoding beta-lactamase resistance in Klebsiella pneumoniae recovered from hospitalized patients in a tertiary care hospital. Methods: A prospective study was conducted in an 1800 bedded tertiary care centre in Pune, India from October 2013 to October 2017. A total of 659 isolates were recovered from clinical specimens of hospitalized patients admitted to the Medical and Surgical intensive care units (one isolate per patient). Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of beta-lactamase encoding genes and conjugation experiments were performed to determine the transferability. Isolate relatedness were determined by REP PCR, ERIC PCR and RAPD. Results: A total of 659 K.pneumoniae isolates were recovered, largest proportion of specimens were from UTIs 30%, followed by 25% in SSTIs, 21.3% in BSIs, 16% in RTIs, and 8% in IAIs and Misc. respectively. Among 659 tested isolates, 32.8% isolates showed MIC >4μg/ml against imipenem and meropenem. Of the total number of samples, males contributed 68.1% while females contributed 31.9%. Of the total samples, highest 28.11% were from OBG, followed by 20.6% in surgery ward, 17.17% in ICU Surgery, 12.46% in Medicine ward and 10% in ICU Medical. Majority of Carbapenem resistant K.pneumoniae 28.83% were from UTIs, followed by 20% in SSTIs, 25.58% in BSIs, 19.4% in RTIs, and 6.51% in IAIs respectively. MHT was positive in 20.7%, DDST in 24.3%, CDST in 26.6%, and MBL (IP/IPI) E-test in 29.3% isolates. 100% K.pneumoniae isolates retained susceptible to colistin. Conjugation experiments indicated that blaNDM-1, blaVIM, blaOXA-48, blaSHV-5, blaSHV-11, blaSHV-12, blaSHV-28, blaCTX-M-15, blaCTX-M-14 were transferable via plasmid. Conclusion: This study highlights prevalence of blaVIM, blaOXA-48 and blaNDM-1, producing K.pneumoniae along with other β-lactamases genes carried on a single or multiple plasmids that serve as a driving force for the horizontal spread of carbapenem resistance. Running title- Metallo-beta-lactamase resistance in Klebsiella pneumonia.

Keywords: BlaNDM-1, blaVIM, blaSHV-5, blaSHV-11, blaSHV-12, blaSHV-28, blaCTX-M-15, blaCTX-M-14, REP PCR, ERIC PCR and RAPD.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)