EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

IN VITRO MICROPROPAGATION OF VANDA ROXBURGHII: A MEDICINAL ORCHID OF THE WESTERN GHATS AND DETERMINATION OF GENETIC FIDELITY USING RAPD MARKERS

Dhananjaya Sasalu Panchaksharaiah*, Gururaj Chalageri and Uddagiri Venkanna Babu

ABSTRACT

Objective: An competent in vitro protocol was developed for rapid multiplication of medicinally and commercially important rare orchid, Vanda roxburghii R.Br., cost effective method was adapted for acclimatization and fidelity test to confirmed genetic similarities. Methods: Different types of media fortified with 10% coconut water (CW) and casein hydrolysate (CH) were used for seed germination as well as PLB formation. MS medium with different auxins i.e. 2,4-D and NAA alone were used for callus induction from leaf explant. Callus were subcultured on Terrestrial Orchid Media (TOM) supplemented with cytokinins and activated charcoal (AC) will yield PLBs and shoot formation. For root development, shoots were subcultured on TOM fortified with BAP along with different concentration of NAA and IAA were analyzed. Results: The maximum seed germination (87%) and PLBs was observed in MS medium fortified with 10% CW and CH 3.28M. Well-developed callus formation was achieved on MS supplemented with 2,4-D (2.27 μM). The PLBs and shoots were observed from the callus on TOM with BAP (8.88 μM) and AC (8.3M). Well-developed root with shoots were observed on TOM fortified with BAP at 8.88 μM with 8.05 μM NAA. The novel brick base method adopted for primary acclimatization and 95% survival frequency were observed. The genetic uniformity was confirmed through RAPD profile. Conclusion: The present investigation provides a commercially viable protocol for this species with adopting novel technique for acclimatization process and field trials. The RAPD results confirms similarity of genome in vitro plants.

Keywords: Acclimatization, indirect regeneration, orchid seed germination, protocorm like bodies, Vanda roxburghi.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)