EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

CHIKUNGUNYA VIRUS INFECTION EVALUATION USING RAPID DIAGNOSTIC TESTS (RDTS) BASED ON ANTI-CHIKUNGUNYA IGM/IGG

Won-Shik Kim, Yong-Gyoo Lee, Man Kyu Huh and Yong Lim*

ABSTRACT

Chikungunya virus (CHIKV) is a single-stranded RNA alphavirus. Recently, CHIKV began an unprecedented global expansion. Thus, it is necessary to develop commercial rapid diagnostic kits for CHIKV infection. We assessed the utility of the commercially available blood tests approved by the rapid diagnostic tests (RDTs) of GenBody Company (Korea) for the detection of CHIKV. In addition, to detect the presence of viral genomic RNA in human samples, RT-PCR of CHIKV was done. RT-PCR of CHIKV was shown the typical banding profile of the rapid diagnostic test for 30 cases of CHIKV negative sera with primers targeting a 557-bp region of the E2 gene. 29 sera were distinct bands (96.6% sensitivity). In this study, the 470 samples were submitted to the Focus Diagnostics Reference Laboratory for CHIKV RNA and/or CHIKV antibody (IgG and IgM). Sensitivity of the GenBody Company RDT was 87.5% (105/120) for IgG and its specificity was 98.8% (316/320). 40 samples were IgM-antibody positive. Sensitivity of the GenBody Company RDT was 74.1% (40/54) for IgM and its specificity was 97.5% (312/320). Most IgM-positive sera were also IgG positive (145/174 = 83.3%). 16.7% were positive for IgM-antibody but negative for ELISA/RT-PCR. It needs to make a new analysis kit such as RNA composition for the detection of anti-chikungunya IgM.

Keywords: Chikungunya fever (CHIKV), IgG, IgM, rapid diagnostic tests (RDTs), RT-PCR of CHIKV.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)