EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (Under Process) , 

 ISSN 2394-3211

Impact Factor: 4.897

 ICV - 79.57

Abstract

DETECTION OF STAPHYLOCOCCI IN MILK SAMPLES IN RETAILS IN KAFR ELSHEIKH GOVERNORATE, EGYPT

Rana Tawfik Abd El-Rahman, Dalia Talat, Treza Mourice Azer and Madiha Salah Ibrahim*

ABSTRACT

The present work was undertaken to study the prevalence, antimicrobial susceptibility and molecular characterization of Staphylococcus aureus in milk samples in retails in Kafr Elsheikh governorate, Egypt. A total of 150 raw milk samples were examined, 66 Staphylococcus isolates were detected (44%). The incidence of S. aureus among a total of 66 samples was 34 isolates with a percentage of 51.51%. The antimicrobial susceptibility pattern of recovered S. aureus isolates revealed that the highest resistance was exhibited against neomycin; 100% and kanamycin; 97.1%. On the other hand, the most effective drugs were oxacillin, ciprofloxacin and cephalotin with bacterial resistance percentages of 91.2%, 82.3% and 79.4%, respectively. PCR was applied to detect mec A gene. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in 40% of chosen S. aureus isolates. Multiplex PCR for Staphylococcal enterotoxin genes (Sea, Seb, Sec, Sed) revealed that genes coding core Sea and Sec were more frequent in a percent of 40% of tested isolates, whereas Seb was 20% and Sed was completely absent. This study indicated that the quality of raw milk is low especially if introduced in non-heat-treated milk products so, more efforts are required to maintain consumer health.

Keywords: Staphylococcus aureus, Antimicrobial resistance, MRSA, mecA, Staphylococcal enterotoxin.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (Under Process)