SPECTROPHOTOMETRIC DETERMINATION OF METHOTREXATE IN TABLET DOSAGE FORM
Samrat Suryawanshi*, Pratiksha Shinde, Nita Thamke, Mukesh Mohite
ABSTRACT
Two simple, precise and economical UV methods have been developed for estimation of Methotrexate in bulk formulation. Method A involves measurement of UV absorbance in Zero order derivative at 258nm. Method B deals with area under curve measurement (AUC method), which involves the calculation of integrated value of absorbance with respect to wavelength between 252-264nm. The drug follows Beer-Lambert’s law in the concentration range of 3-15μg/ml in both the methods. Results of analysis were validated statistically and were found to be satisfactory. Thus proposed methods can be successfully applied for estimation of Methotrexate routine analytical work.
Keywords: Methotrexate, Zero order derivative, Area Under Curve method (AUC), UV Spectrophotometer.
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