IMMUNOHISTOCHEMICAL STUDY OF CD15 EXPRESSION IN CD30+ CLASSICAL HODGKIN'S LYMPHOMA

Kafil Akhtar M.D., *Thaeer Jawad Kadhum, Aws Rassul hussain, Mohd Rafey, Wasim Rizvi, M.D.,  Rana K Sherwani, M.D., 

ABSTRACT

Background: Hodgkin lymphoma (HL) is a histologically defined B-cell neoplasm that includes two distinct types of disease, classical and nodular lymphocyte-predominant. Aim of the study: To evaluate the immunohistochemical expression of CD15 in CD30+ classical Hodgkin’s lymphoma. Material and Methods: A retrospective study on 52 diagnosed cases of HL at Al-Yarmouk Teaching Hospital, Baghdad Medical City and Al-Atheer private laboratory. All biopsies were formalin-fixed, paraffin embedded and cut into 4 micron sections and stained with hematoxylin and eosin and CD15 monoclonal antibody(DAKO). Cases with IHC CD30 positive reactivity were included in this study. Results: Cervical lymph nodes were most commonly involved in 35 cases (67.5%). Male: Female ratio was 1.6:1. Age of the patients ranged from 4-80 years with a mean age of 29 years. The study revealed CD15 positive classical HL in 24 cases (75%). Forty (77%) out of 52 of the CD30 positive cHL cases were CD15 positive. Conclusions: All cases of classical Hodgkin’s Lymphoma should undergo immunophenotypic analysis for CD15 in addition to CD30 antigen.

Keywords: Hodgkin's Lymphoma, Immunohistochemistry.