STUDY OF ANTIBIOTIC SENSITIVITY PATTERN AND EXTENDED-SPECTRUM BETA-LACTAMASES DETECTION OF ISOLATED ENTEROBACTERIACEAE

Mohd Nadeem, Umar Farooq* and Vasundhara Sharma

ABSTRACT

Introduction: ESBL producers are one of the most common causes of nosocomial pathogens, which are responsible for causing variety of human infections. In the present era of antibiotic resistance, the emergence of multi-drug resistant organisms is more common. Aims: To observe the antibiotic sensitivity pattern of Enterobacteriaceae and result of Ceftazidime and ceftriaxone for ESBL detection. Provide appropriate list of sensitive antibiotics to clinician for treatment. Materials and Methods: Total 200 isolates of Enterobacteriaceae had been taken from the different types of samples. All the Enterobacteriaceae isolates from different samples received from hospital for gram staining, manual culture, automated bacterial culture and antibiotic susceptibility testing will be included in the study. Results: There were 200 different isolates of Enterobacteriaceae. 174(87%) ESBL producers and 26(13%) non-ESBL producers were isolated. Among the ESBL positive cases of the all samples E.coli (105) was the top most organism followed by Klebsiella spp. (26), Citrobacter spp. (25), Enterobacter spp.(25) and proteus spp. (5). Conclusion: ESBLs have evolved greatly over the last years. There is no doubt that the ESBLs will become increasingly complex and diverse in the future. This will create increasing challenges for those creating guidelines for the detection of ESBLs in the clinical microbiology laboratory. The increasing prevalence of antibiotic-resistant bacterial infections seen in clinical practice stems from antibiotic use within medicine. In medicine the major problem of the emergence of resistant bacteria is due to misuse and overuse of antibiotics.

Keywords: Extended Spectrum Beta-lactamases, Antibiotic Sensitivity Testing.