PHENOTYPIC CHARACTERIZATION OF ACINETOBACTER FROM VARIOUS CLINICAL SAMPLES AND MOLECULAR STUDY ON IMIPENEM RESISTANT STRAINS IN A TERTIARY CARE HOSPITAL, ENATHUR, KANCHIPURAM.
*Dr. M. Anuradha, Dr. K. Muthulakshmi, Dr. Somasunder
ABSTRACT
Background: Acinetobacter is a Gram negative nosocomial pathogen, incapable of causing infection on its own,
however causes colonization in susceptible patients especially with underlying pathology such as neutropenia or
organ transplants or CAPD or catheter induced bacteruria. It is one of the causative agent of nosocomial pneumonia.
A study is done on its causative role among various infections like UT infections, wound infections, respiratory tract
infection, Blood stream infections, for a period of 1year duration. Antibiotic susceptibility Pattern studied and by
using PCR technique imipenem bla OXA genes were studied. Materials and methods: Acinetobacter is identified
by various Biochemical tests such as sugar fermaentation, indole test, methyl red, VP, Citrate utilization test, TSI,
Urease test, mannitol motility. Further studied for Antibiotic susceptibility by using Muller – Hinton agar and Kirby
bauer method and molecular characterization by PCR. Results: Among 1665 urine samples received over a period
of 1 year duration 523 (31.41%) were culture positive, among culture positives commonest isolate was Escherichia
coli 230(43.97%), citrobacter was least common being 6 out of 523 where as Acinetobacter were 16/523
(3.05%).Among pus samples 553 were total samples 409 were culture positive(73.96%),Staphylococcus aureus
173/409 (42.29%) was being the most commonest causative organism and Entercoccus was least common
4/409(0.997%), Acinetobacter were causative agents for 8/409(1.95%).Among sputum samples total nuber were
1313 and culure positive were 512 (38.99%). Acintobacter was isolated from 15 cases (nosocomial) 15/512(3%).
Among various fluids there were 31 isolates among 137 sample (19/147) however, Acinetobacter was not isolated
and among blood stream infections 3/386 cases(0.77%) were positive for acinetobacter. Conclusions: Among
various samples Acinetobacter was causative agent for only few casaes of 1 – 3% however common among
hospital acquired infections.2 strains were resistant to imipenam and 2 strains were moderaetly resistant to
imipenam .The present study shows their greater incidence among women with age group (50-60). Imipenem
resistant strains were studied for blaOXA genes.
Keywords: Acinetobacter, pathology, using PCR technique imipenem bla OXA genes.
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