IN-VITRO OPTIMIZATION PROTOCOL OF WHEAT CULTIVARS IN NEWLY ESTABLISHED LAB OF PLANT TISSUE CULTURE, MUZAFFARABAD

Safoora Ishfaq, Syed Dilnawaz Ahmed, Asad Hussain Shah, Rizwan Taj Khan, Syeda Maria Fiaz Bukhari, Isma Hameed, Hira Mubeen, Nazish Awan, Syed Rizwan Abbas and Shahid Raza*

ABSTRACT

The experiment was conducted to check the sterilization effect of different concentrations of Clorox on six wheat varieties. This experiment was conducted in plant tissue culture lab of biotechnology, university of AJK. Seeds of selected wheat cultivars were used as an explant and surface sterilized with different (30%, 50% and &70%) concentrations of Clorox. After sterilization they were washed three times with double distilled water for 10 minutes. Different concentrations of Clorox effected differently the seeds. At 30% Clorox maximum germination was observed by the variety Galaxy-01 (16.6625%). Chakwal-50 (12.5%) and Borlog-14 (12.5%) showed the same germination at 30% Clorox. NARC-09 (8.25%) and Millet-11(8.25%) showed the same results and Pakistan-13 (0%) showed no germination at 30% Clorox. Maximum germination at 50% Clorox was shown by the variety Galaxy-01 (29.1625%) and Millet-11 (29.1625%). NARC-09, Pakistan-13 and Chakwal-50 showed 20.825%, 20.825% and 16.6625% germination respectively. Less germination was shown by the variety Borlog-14 (4.1625%). At the concentration of 70% Clorox the maximum germination was shown by the variety Borlog-14, which is 37.5%. Millet-11 showed 25% germination. NARC-09 and Pakistan-13 showed no germination at 70% Clorox. Less germination was recorded by the variety Galaxy-01(12.5%). The results indicated that all varieties behaved differently at different levels of concentrations. It is concluded that for sterilization of wheat seeds the 50% Clorox is better than other concentrations used.

Keywords: Galaxy-01 (16.6625%), NARC-09 (8.25%) and Millet-11(8.25%).